Jong-Young Kwak
Jong-Young Kwak, MD/PhD
Department of Pharmacology & Immune Network Pioneer Research Center,
Ajou University School of Medicine,
164, World cup-ro, Yeongtong-gu, Suwon 443-380,
T: +82-31-219-5064
F: +82-31-219-5069
M: +82-10-8514-7487


Dr. Kwak is professor at Department of Pharmacology, Ajou University School of Medicine, Korea. He is currently the Director of Immune-network Pioneer Research Center, which is sponsored as one of the Pioneer Research Center Program by the Korean ministry of Science ICT & Future Planning. He became a vice-president of Korean Society of Biochemistry and Molecular Biology in 2015 and is a Doctor Honoris Causa in Russian Academy of Science since 2012. He completed his doctorate in Medical Biochemistry with neutrophil activation and signal transduction pathways at the Pusan National University, Korea in 1991. After his study of activation of neutrophils in Emory University as a post doctorate, he directed his research to dendritic cell analysis. Current research topics in his laboratory are dendritic cell regulation, imunogenic response of damaged cells, and 3D culture of immune cells.


Biomimetic immune cell network in nanofibrous scaffold-based 3D culture system

Effective inflammatory and immune processes are initiated after recognition of microbial invasion or damaged host tissues by immune cells. First, we developed a 3D multicellular culture model for cancer and immune cells to form a more physiologically relevant representation of the cancer microenvironment than currently available 2D systems. Cancer cells and dendritic cells (DCs) deeply infiltrate the 3D nanofibrous scaffold (NFS), allowing observation of their interactions. Direct addition of DCs to mitoxantrone-treated cancer cells cultured in 3D NFS enables DCs to spread, migrate, and adhere to the cancer cells in a 3D manner. Second, we developed a 3D in vitro infection model to investigate the cross-talk between phagocytes and microbes in inflammation. S. aureus and phagocytes, including neutrophils, macrophages, and DCs are able to adhere to nanofibers inside the NFS and phagocytes migrate to and engulf S. aureus in a 3D manner. 3D NFS system has some advantages in investigating an immune cell network; easy observation of the movement of phagocytes over time, evaluation of 3D migratory behavior of immune cells, quantitative evaluation of the rates of migration of phagocytes and the phagocytosis of bacteria. Thus, NFS-based 3D culture system is widely applicable to the biomimetic study of various immune cell responses to microbe infections and damaged host cells.
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  September 15, 2017